Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: In Vivo Tumor Angiogenesis Imaging Using Peptide-Based Near-Infrared Fluorescent Probes

doi: 10.1007/978-1-4939-3721-9_8

Figure Lengend Snippet: Confocal microscopy results of Cy5.5-NGR2 with HT-1080 cells (CD13 positive) and MCF-7 cells (CD13 negative) (magnification: 100×). The blocking study is achieved by adding unlabeled monomeric NGR peptide. Top: Incubation of Cy5.5-NGR2 (20 nM) with CD13-positive HT-1080 cells; middle: incubation of Cy5.5-NGR2 (20 nM) with CD13-positive HT-1080 cells blocked by a non-labeled monomeric NGR peptide (50 μM); bottom: incubation of Cy5.5-NGR2 (20 nM) with CD13-negative MCF-7 cells. Reproduced from Ref. 6 with permission from Springer

Article Snippet: list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Generate the tumor xenografts by subcutaneous injection of five million HT-1080 cells suspended in 50 μL of cell culture media and 50 μL of BD Matrigel into the right shoulder of female athymic nude mice (Harlan Laboratories, Livermore, CA, USA) ( see Note 9 ).

Techniques: Confocal Microscopy, Blocking Assay, Incubation, Labeling

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: In Vivo Tumor Angiogenesis Imaging Using Peptide-Based Near-Infrared Fluorescent Probes

doi: 10.1007/978-1-4939-3721-9_8

Figure Lengend Snippet: Time-course fluorescence imaging of subcutaneous HT-1080 tumor-bearing nude mice after intravenous injection of 1.5 nmol of Cy5.5-NGR2. The tumors are clearly visualized as indicated by an arrow from 0.5 to 4 h pi. The fluorescence intensity is recorded as per second per centimeter squared per steradian (p/s/cm2/sr). Reproduced from Ref. 6 with permission from Springer

Article Snippet: list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Generate the tumor xenografts by subcutaneous injection of five million HT-1080 cells suspended in 50 μL of cell culture media and 50 μL of BD Matrigel into the right shoulder of female athymic nude mice (Harlan Laboratories, Livermore, CA, USA) ( see Note 9 ).

Techniques: Fluorescence, Imaging, Injection

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: In Vivo Tumor Angiogenesis Imaging Using Peptide-Based Near-Infrared Fluorescent Probes

doi: 10.1007/978-1-4939-3721-9_8

Figure Lengend Snippet: Quantification and kinetics of in vivo targeting character of Cy5.5-NGR2 in the HT-1080 tumor vs. muscle. The Cy5.5-NGR2 uptake in HT-1080 tumor at various time points is significantly higher than that in muscle. Error bar is calculated as the standard deviation (n = 3). Reproduced from Ref. 6 with permission from Springer

Article Snippet: list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Generate the tumor xenografts by subcutaneous injection of five million HT-1080 cells suspended in 50 μL of cell culture media and 50 μL of BD Matrigel into the right shoulder of female athymic nude mice (Harlan Laboratories, Livermore, CA, USA) ( see Note 9 ).

Techniques: In Vivo, Standard Deviation

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: In Vivo Tumor Angiogenesis Imaging Using Peptide-Based Near-Infrared Fluorescent Probes

doi: 10.1007/978-1-4939-3721-9_8

Figure Lengend Snippet: Representative optical imaging (at 2 h pi) of mice bearing HT-1080 tumor on the right shoulder demonstrating blocking of Cy5.5-NGR2 (1.5 nmol) uptake by co-injection with a non-labeled monomeric NGR peptide (20 mg/kg). Reproduced from Ref. 6 with permission from Springer

Article Snippet: list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Generate the tumor xenografts by subcutaneous injection of five million HT-1080 cells suspended in 50 μL of cell culture media and 50 μL of BD Matrigel into the right shoulder of female athymic nude mice (Harlan Laboratories, Livermore, CA, USA) ( see Note 9 ).

Techniques: Optical Imaging, Blocking Assay, Injection, Labeling

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: In Vivo Tumor Angiogenesis Imaging Using Peptide-Based Near-Infrared Fluorescent Probes

doi: 10.1007/978-1-4939-3721-9_8

Figure Lengend Snippet: Fluorescence intensity ratio of tumor to muscle based on the ROI analysis of Cy5.5-NGR2 uptake at 2 h pi in HT-1080 tumors without (non-blocking) or with (blocking) co-injection of a non-labeled monomeric NGR peptide (20 mg/kg). Error bar is calculated as the standard deviation (n = 3). Reproduced from Ref. 6 with permission from Springer

Article Snippet: list-behavior=enumerated prefix-word= mark-type=decimal max-label-size=0 Generate the tumor xenografts by subcutaneous injection of five million HT-1080 cells suspended in 50 μL of cell culture media and 50 μL of BD Matrigel into the right shoulder of female athymic nude mice (Harlan Laboratories, Livermore, CA, USA) ( see Note 9 ).

Techniques: Fluorescence, Blocking Assay, Injection, Labeling, Standard Deviation